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Therefore, considering the presence of APAP protein adducts at the therapeutic dose of APAP, formation of protein adducts on mitochondrial proteins rather than the overall formation of protein adducts may be the reason for cellular toxicity, which has been considered a key factor for necrotic cell death in previous years (Jollow et al., 1973

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To confirm the metabolic changes, we next performed targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) analysis of plasma and ovarian tissues