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They argued that the FDAs decision to declare the shortages resolved was reckless and arbitrary, alleging the agency relied solely on manufacturer assurances of supply while patients still faced inability to fill prescriptions.22 Outcome: In March 2025, Judge Mark Pittman denied the OFAs motion for a preliminary injunction regarding tirzepatide, and subsequently for semaglutide in April

Merck Millipore, Darmstadt, Germany) tubes and centrifuged at 75,000 g for 40 min at 4 C

Over time, p53 outside the nucleus may become unstable and start to aggregate, and tauO near the nucleus interacts with p53 to form a p53 oligomer

FASEB bioAdv (2019) 1(2):6780