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Nystatin (Sigma-Aldrich) was prepared in dimethyl sulfoxide (DMSO

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Abbreviations ACE: angiotensin-converting enzyme ACh: acetylcholine ADMA: asymmetric dimethyl-L-arginine ATII: angiotensin II BH 4 : tetrahydrobiopterin cGKI: cGMP-dependent protein kinase cGMP: cyclic guanosine monophosphate CTP CH-I: GTP cyclohydrolase I DDAH: dimethylarginine dimethylaminohydrolase DETC: diethylthiocarbamate DHFR: dihydrofolate reductase DOCA: deoxycorticosterone acetate eNOS: endothelial nitric oxide synthase FMD: flow-mediated dilation LDL: low-density lipoprotein L-NAME: N G -nitro-L-arginine methyl ester NO: nitric oxide NTG: nitroglycerin ONOO : peroxynitrite PDE1A1: phosphodiesterase 1A1 PKC: protein kinase C PRMT: protein arginine N -methyltransferase ROS: reactive oxygen species sGC: soluble guanylyl cyclase SHR: spontaneously hypertensive rats SNP: sodium nitroprusside SOD: superoxide dismutase STZ: streptozotocin VASP: vasodilator-stimulated phosphoprotein

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