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(100) shiitake mushrooms were exposed to intermittent and single ozone treatments, then stored at a temperature of 4C for a period of 14 days with 90% relative humidity demonstrating that periodic application at the ideal dosage of 3.2 mg/m 3 outperformed the single treatment preserving proteins and phenolic compounds, inhibiting polyphenol oxidase activity to inhibit the occurrence of surface discoloration and decreasing the accumulation of unbound amino acids within the mushrooms

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Glutathione oxidation-reduction (redox) cycle The figure illustrates the glutathione oxidation-reduction (redox) cycle