The lymphocytes isolated above were subjected to immunofluorescence staining with Rat FITC, APC, PE (BD Pharmingen, USA), and labeled anti-rat CD3 + , CD4 + , and CD8 + monoclonal antibodies, respectively, reacting at 4C for 30 min in the dark
10.1161/HYPERTENSIONAHA.110.156554 115 MassiminoE.IzzoA.RiccardiG.PepaG
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