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Whereas, vitamin C deficiency is associated with bleeding dysfunction at the level of platelet adhesion to collagen and is, therefore, measurable with an associated increase in bleeding time, vitamin K deficiency has no effect on bleeding time

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Western blot analysis The kidney organoids were homogenized in boiling lysis buffer (1% SDS, 1 mM sodium orthovanadate, and 10 mM Tris, pH 7.4), and the protein concentration was determined with a BCA Protein Assay Kit (Pierce Biotechnology Inc., Rockford, IL, USA)

PMID 15004691