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When MDA5 detects viral dsRNA, the CARD domains undergo ISG15-mediated ISGylation, facilitating MDA5 oligomerization onto the RNA and subsequent CARD clustering and binding to MAVS (104)

For the assessment of amino acid selectivity of these electrophiles, we implemented an LC-MS/MS-based assay 37 utilizing a nonapeptide (KGDYHFPIC) having multiple nucleophilic residues (Cys, Lys, Tyr)

Zhu J, Yang Q, Li H, et al