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Isolation of total RNA and cDNA synthesis Total RNA was isolated from liver tissue with ISOGEN (Nippon Gene Co., Ltd., Tokyo, Japan) according to the manufacturers instructions

Opposing associations of NT-proBNP with risks of diabetes and diabetes-related complications

As detailed in the Methods section and legend, infected cell lysates were passed over DDX3X fragments immobilized on Sepharose beads and analysed by Western blotting to see if pU L 31 binds to these fragments

Immediate management follows the "Rule of 15": consume 15 grams of fast-acting carbohydrate (4 glucose tablets, 4 ounces of fruit juice, or 1 tablespoon of honey), wait 15 minutes, and recheck blood glucose

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