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H 2 O 2 reacts with potassium iodide (KI) to produce I 2 /I 3 , which can be quantified by measuring the characteristic absorption peak at 350 nm using UVVis spectroscopy [33]

This early work on hepatocytes subsequently extended to rodent cell lines and various animal models, establishing a foundation for decades of research (Pickart, 1977)

*Final cost depends on BMI grading, treatment zones, and clinical complexity

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Both variants had similar kinetic parameters as the WT, indicating that though catalytically unchanged, the stability of these variants is markedly reduced, with G6PD Plymouth present in even lower amounts and ultimately leading to more severe clinical manifestations