Isolated hepatocytes were plated on collagen-coated 6-well plates (4 10 4 cells per cm 2 ) in DMEM (Gibco #12800-017, containing 1 mM pyruvate, 25 mM glucose, and 4 mM glutamine) supplemented with 10 % (vol/vol) fetal bovine serum (FBS), 100 units/ml penicillin, 100 g/ml streptomycin, 1 M dexamethasone, 0.1 M insulin, and an additional 1 mM sodium pyruvate and incubated at 37 C in an atmosphere of 5 % CO 2
If your body has trouble absorbing B12, you might get daily B12 supplementation shots for around two weeks at first until you feel better
10.1016/j.lfs.2020.118627 236 KhanM
doi: 10.1371/journal.pntd.0003100
Many lower vertebrates including fish, frogs, and birds have both AGAT and GAMT activities in their livers and kidneys, which is in contrast to mammalian and avian species [28, 29]