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To detect Ki-67 binding, a biotinylated goat anti-rabbit secondary antibody was used at 1:250 dilution for 30 minutes, followed by the ABC reagent (Vector, AK-5000) for 5 minutes

[DOI] [PubMed] [Google Scholar] 197.Zhang Y., Cartland S.P., Henriquez R., Patel S., Gammelgaard B., Flouda K., Hawkins C.L., Rayner B.S

This included: Reconciling top-down and bottom-up estimates Cross-checking revenues, volumes, and pricing assumptions Incorporating expert insights to validate segment splits and adoption rates This multi-angle validation yielded the final market size

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Then 100 l of Fura-2 AM solution was added to the biofilms and incubated for 1 h at room temperature