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While the correlation between low GSH levels or low GSH:GSSG ratios and aging seems clear, the precise reasons for the age related change in the content of GSH is less well characterized

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In the RT structure, the cyano group at the position of the Michael acceptor enables fast reaction kinetics, the four-membered azetidine ring improves quantum yield and photostability 17 , and the two carboxylic acid groups ensure aqueous solubility and reduce probe binding to hydrophobic cellular structures

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It must survive stomach acid and digestive enzymes, then be absorbed through the intestinal lining into the bloodstream