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RICCS cross-correlation analysis of Laurdan-labelled lipid co-diffusion with GLP-1R WT versus V229A demonstrated a reduction in the movement of the GLP-1R-lipid complex for WT receptors after exendin-4 stimulation, with a tendency for V229A receptors to exhibit the same effect under vehicle conditions, resulting in no significant change for GLP-1R V229A-lipid complex diffusion between vehicle and exendin-4-stimulated conditions (Figure 4E, Supplementary Figure 2D)

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