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However, the argument was refuted for the strain Z8 when grown in a chemically defined medium (CDM) and it was found only to accumulate GSH from the medium [19]

Physical enhancement methods including iontophoresis, microneedling, and ultrasound also significantly increase GHK-Cu delivery, with microneedling pre-treatment increasing peptide penetration by approximately 10-fold compared to intact skin application, effects similar to those achieved with other topical peptide formulations optimized for transdermal delivery

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