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Gallbladder-related complications, including cholelithiasis and cholecystitis, are coded within the K80-K82 range

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2.6 Real-time PCR analysis Total RNA was prepared from liver tissues and LO2 cells using the simple RNA Extract kit (Tiangen Biotech, China) and reverse transcription of RNA to cDNA using Fast King cDNA First Strand Synthesis Kit (Tiangen Biotech, China)