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These include direct inhibition of inflammasome components, caspase-1 blockade, modulation of immune responses using anti-inflammatory agents, and targeted manipulation of gut microbiota and bile acid metabolism, which are increasingly recognized for their roles in liver homeostasis and injury response

Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts

PT-141, also known as Bremelanotide, acts as a melanocortin receptor agonist that stimulates melanocortin receptors in the brain associated with sexual desire and arousal

This makes oral delivery the preferred route for GI-specific indications, while injectable delivery is preferred for systemic tissue repair goals (joint, muscle, tendon, systemic inflammation)

Values are represented as mean SE