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Additionally, we established an indirect ELISA using the rSmGST
This is partially because men typically carry more visceral fat, which responds quickly to the metabolic changes GLP-1 creates
On January 14 th , Monte Nido Vice President of Adolescent Medicine Rebecka Peebles, MD, FAAP, CEDS, DABOM
To determine if GLP-1R induces cAMP generation specifically at VAPB locations, we modified a fluorescence resonance energy transfer (FRET)-based system of biosensors targeted to endogenous proteins (FluoSTEPs) 25 , so that a FRET cAMP sensor would fully reconstitute only in the presence of the GFP 11 fragment fused to VAPB, resulting in functional biosensor assembly specifically at VAPB loci (Supplementary Fig
This matches official guidelines (for example, Hospiras package insert)
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