Isolated CD4 + and CD8 + T cells were activated or not for up to 1, 3, or 6 days in a reverse time course with Dynabeads (1:1 cell-to-bead ratio, Gibco), magnetic beads similar in size to antigen-presenting cells and coupled with anti-CD3 and anti-CD28 antibodies, so that their total time in culture would be identical and to ensure that all cells would finish their indicated activation period the same day
Tartsa a dobozt prtl, nedvessgtl, napfnytl vdve, jl lezrva, hvs helyen, kisgyermektl elzrva!
Endocrinology 140: 11321140
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