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Lean proteins provide amino acids without adding fermentation burden

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Briefly, cells were cultured in DMEM/F12 supplemented with 10% FBS, 100 U/ml penicillin, 100 mg/ml streptomycin, 8 g/ml biotin, and 4 g/ml pantothenic acid (all from Thermo Fisher Scientific Waltham, MA, United States)