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Membranes were incubated overnight at 4 C with a range of 1:10002000 dilutions of AMPK, pAMPK, ACC, pACC, TRPA1, IL-1, TNF-, cyc, and -actin primary antibodies, followed by HRP-conjugated secondary antibodies
This is not about self-diagnosing
In pregnancy, ROS are generated mainly in the placenta, but xanthine oxidase (XO) are present in the vascular endothelium, and nitric oxide synthase (NOS) are also sources of ROS [15]
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