For GSSG, the samples were treated with 2-vinyl pyridine (2-VP, 0.2% v/v beforehand), which covalently binds with GSH, removing it entirely, thus leaving GSSG as the only measurable substrate of the assay
Nurjanah, Kresnawati L, Mufid A
After de-paraffinization, they were stained with hematoxylin and eosin (H&E)
If You Experience Problems If you experience concerning symptoms-severe abdominal pain, persistent vomiting, signs of allergic reaction, or other significant issues-contact your prescribing physician or seek immediate medical attention as appropriate
Leptin regulates gastrointestinal satiation signals, such as gastric distension, CCK or glucagon-like-peptide-1 (GLP-1) by acting on LepR in mNTS/AP neurons [56], [57], [58], [59]