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5gh), and differs significantly from the yeast-produced SC6b D particle (RMSD across all pairs = 1.976 )

Immediately after fixation the samples were washed once briefly with MilliQ and stained overnight at 37C with fresh SA--GAL solution (pH 6.0), containing 2,5 mM Na2HPO4, 7,4 mM Citric Acid, 0.15M NaCl, 2mM MgCl2, 5 mM Potassium Ferricyanide, 5 mM Potassium Ferrocyanide and 25ul/ml 4% X-gal in DMF

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