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Using a syringe pump (KD Scientific, MA, USA), we bilaterally microinjected 0.8 l of virus mixture into either the IL (dorsoventral [DV], 3.2 mm from the bregma) or PL cortex (DV, 2.2 mm from the bregma) at a rate of 0.1 l/min

Given that FXS is a monogenic cause of ASD, these findings highlight both the challenges of translating GABAergic modulation into broad clinical efficacy and the importance of patient stratification in future trials of targeted therapies for neurodevelopmental disorders (152)