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Finally, considering the potential application of these thioamide-derived warheads in TCIs, we performed in vitro human liver microsome metabolism experiments and assessed the remaining compounds by HPLC measurements (Supplementary Fig

This clarification step is crucial to prevent clogging of the affinity column and to enhance the efficiency of the purification process

doi: 10.1038/cr.2009.108 130 WuF.GaoY.YangW.SuiN.ZhuJ

This further leads to the reversal of mitochondrial dysfunction, thereby increasing mitochondrial -oxidation and reducing intracellular oxidative stress to prevent hepatic lipotoxicity (53, 54)