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Antioxidant enzymes such as superoxide dismutase (SOD) play a key role in diminishing oxidative stress

To further characterize these interactions, we constructed a molecular interaction network (Figure 7C) revealed three functionally interconnected modules: ( Consistent with multi-omics findings, KEGG pathway analysis identified glutathione metabolism as the most significantly perturbed shared pathway (Figure 8A), exhibiting coordinated changes between upregulated antioxidant enzymes such as Gpx3 and Mgst1 alongside depleted glutathione derivatives, supporting redox imbalance as a key pathogenic feature

Our results suggest that the responses to individual stress factors do not merely aggregate to elicit barleys reaction to dual stress

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