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To construct the sorting vector for gene overexpression, we amplified the whole expression cassette of N-signal-GST-EGFP-GPI DAF and N-signal-GST-EGFP-GPI CEAM7 from pEGFP-GST-GPI DAF and pEGFP-GST-GPI CEAM7 vectors and joined them with PCR linearized pcDNA3.1 vector using ClonExpress II One Step Cloning Kit

Lignans from flaxseed, converted to enterolactone (EL) and enterodiol by gut microflora within the intestines, possess weak estrogenic activity that competes with endogenous oestrogens for ER binding causing decreased mitogenic stimulation and lower circulating estrogen concentrations via upregulation of sex hormonebinding globulin (SHBG) (189, 190)

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Chemotherapy-induced liver injury: unveiling emerging mechanisms and exploring mitigation strategies