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Equivalent amounts of cell lysates were separated using SDSPAGE and transferred to PVDF membranes

In brief, the sections were dewaxed and incubated in Bouins solution overnight at room temperature and then stained with aniline blue and Scarlet to visualize the collagen fibers and nuclei, respectively, under dark conditions at 4 C

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As biofilms can increase the antibiotic resistance of bacteria up to 1,000-fold (Hiby et al., 2010), therefore the dissolution or prevention of these biofilms is an important part of the host antimicrobial defense and presents a challenge to both the host immune system and also the therapeutic development