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Subsequently, HaCaT keratinocytes viability after treatment with test compounds, GHK, CuCl 2 and Cu(OAc) 2 were assessed using MTT assay and LDH assay

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However, the efficiency of the ATP supply or utilization for GSH production seems to be very low because only 0.5 % of the total ATP regenerated in the glycolytic pathway is used for GSH production under anaerobic conditions [13]

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Enzymatic antioxidant response is carried out by superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) related enzymes such as glutathione peroxidase (GPx) [92]