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Flow cytometry analysis The cells from the cell sorting procedure were directly subject to flow cytometry analysis using NovoCyte Flow Cytometer (ACEA Bio)

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Overview of persistent pain in older adults

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(4) Centrifuge for 10 min (12000 rpm, 4C), aspirate 150 L of the supernatant with a syringe, filter it using a 0.22 m organic phase pinhole filter, transfer it to an LC injection vial and store it at -80C until LC-MS analysis