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J Neurosci 33:89235

One unit of GST activity was equal to the amount of enzyme that catalyzed the transformation of 1 nmol of substrate into the product within 1 min per mg of protein (mU/mg)

Cell Culture HepG2 cells were cultured in collagen-coated plates and maintained in DMEM containing 10% FBS, 1% penicillin/streptomycin (PAA Laboratories, Austria), 1% ultraglutamine (Lonza, Switzerland) and 1% non-essential amino acids (Sigma-Aldrich, Germany)