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Protein precipitation using methanol (Merck KGaA, Darmstadt, Germany) and 0.1% formic acid (Merck KGaA, Darmstadt, Germany) was performed and the collected supernatant was treated with a derivatization solution of aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) (Merck KGaA, Darmstadt, Germany)
This is simply because there is less colored liquid for light to pass through
One of the mechanisms proposed for melanocyte death is the oxidative stress hypothesis
Trimethylated Quaternary Ammonium Group The quaternary ammonium group (N+(CH3)3) is a positively charged nitrogen atom bonded to three methyl groups