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Oral administration of 10 mg/kg of EGCG from a presymptomatic stage delays the onset of the disease and prolongs useful life, in addition to increasing the number of motor neurons, decreasing the activation of the microglia, reducing the concentration of NF-kB, caspase-3 and iNOS in a transgenic mouse model of ALS (Xu et al., 2006) (Table 1)

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Each time a cell divides, telomeres naturally shorten, eventually reaching a critical length that triggers cellular senescence or death

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Then, add 20 L of the sample and diluted standards into the detection well and measure the relative light unit (RLU) using a SpectraMax 190 full wavelength spectrometer (Molecular Devices Corporation), and ATP level then was calculated based on the standard curve