[U- 13 C]glutamine isotope tracing experiments showed that GGCT overexpression reduced Gln contribution to the TCA cycle and diverted it preferentially to the GSH synthesis pathway for ROS regulation
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The cells were then washed once in ice-cold PBS, trypsinized, pelleted, and then homogenized in mitochondrial isolation buffer (0.21 M mannitol, 0.07 M sucrose, 0.1 mM EDTA, 1 mM EGTA, 10 mM Tris HCl, 0.5% BSA, pH 7.4 using KOH)
250 mcg Other Ingredients: Vegetable Capsule (Hypromellose), Microcrystalline Cellulose, Vegetable Stearate Warnings/Cautions: Consult your healthcare practitioner prior to use if you are pregnant, nursing, taking medication, or have a medical condition