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While the results were encouraging, by the mid-1990s Holst and Carolyn Deacon, a physiologist at the University of Copenhagen, confirmed an earlier finding by Rolf Mentlein, Baptist Galllwitz and Wolfgang Schmidt based at the University of Kiel in Germany who had found in 1993 that GLP-1 did not last long in the body because it was broken down in minutes by dipeptidyl peptidase-IV (DPP-4), an enzyme found in numerous tissues and in the bloodstream (Mentlein, Galllwitz, Schmidt

Using metformin alone can activate the AMPK pathway and inhibit the AKT pathway, while using simvastatin alone only inhibits the AKT pathway

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Acknowledgements We thank Wolfgang Kabsch for helpful discussions as well as Marina Fischer and Dr Rimma Iozef for technical support

Storage temperature Moisture exposure Light exposure Handling frequency Packaging integrity Why Lyophilisation Matters Removing water through freeze-drying helps reduce degradation pathways that may occur in solution