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Clinical trials are testing a 25 mg dose, which has shown weight loss results comparable to the highest dose of Wegovy, its injectable counterpart for weight loss

After 24 h, wells were co-transfected with 1.1 g full-length FLAG-tagged PTEN in pCDNA3.1 vector (Addgene #22231) and 1.1 g N-terminal OSBP pleckstrin-homology domain tagged (to promote colocalization with PTEN), C-terminal 6His-tagged proteases in pCDNA3.1 using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) following the manufacturers instructions

Allergic reactions can occur, most often to thiamine (vitamin B1)