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Evaluation of cell apoptosis by Annexin V assay To investigate the possible role of MVs treatment on apoptosis induction in fibroblasts, on days 1, 3, and 5 of culture, Annexin V-FITC and PI (EXBIO, Vestec, Czech Republic) were utilized for double-staining of cells, followed by flow cytometry (BD Biosciences, San Jose, CA, USA) analysis

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The culmination came with Wegovys June 4, 2021, approval of semaglutide 2.4 mg specifically for chronic weight management

Based on these criteria, the incidence of thyroid dysfunction induced by pembrolizumab was 8.1%, which was similar to that induced by nivolumab (10.2%)