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Membranes were blocked with 5% dry milk in Tris-buffered saline Tween-20 for 1 h at room temperature and subsequently incubated overnight with primary antibodies at 4 C

Emeville, E

[315] Comment: A few commenters recommended stratification by ED volume, with a commenter suggesting stratifying ED volume as small, medium, or large, to enable meaningful comparisons across hospitals with varying patient volume and resource levels

For others, cannabis use may reduce antidepressant effectiveness, potentially leading to persistent or worsening symptoms

It has not been tested or approved by the FDA and could lead to serious health consequences if used improperly