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For the orthotopic pancreatic cancer model, VRK2-WT or VRK2-KO Pan02 cells in logarithmic phase (8090% confluence) were harvested, detached with 0.25% trypsin-EDTA, centrifuged (1000 rpm, 5 min), and resuspended in cold serum-free RPMI-1640 medium

One weekly administration, IGF-1 maintained at plateau for up to 28 days

Jakhar D, Sarin SK

The variation between analyses of material from the same filter may indicate that RuBisCO was not homogeneously distributed throughout the sample that may contribute a significant number of highly effective INPs active at warm temperatures