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It has been demonstrated that basophils can regulate the entry of eosinophils into sites of inflammatory tissue through activation-induced interactions with endothelial cells [134]

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Briefly, cells were labelled with 1 M BG-S-S-649, a cleavable SNAP-Surface probe, in full media for 30 minutes at 37C, washed with PBS and stimulated with vehicle or 100 nM exendin-4 in reverse time order for 30, 15, 10 and 5 minutes at 37C in full media

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