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The assay mixture consists of 1 mL of 50 mM phosphate buffer with 0.4 mM EDTA (pH 7.0) and contains 0.2 mg liver homogenate protein
For the various modeled conditions, we applied literature-based scaling factors to the concentrations of enzymes and transporters, as well as initial concentrations of variable metabolites
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For chronic hypoxia, mice were treated continuously with 13% O 2 for 1, 3, 7, and 14 days