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Sulforaphane protected against rotenone neurotoxicity via modulating autophagy Recent reports indicate that the pathology of PD is closely related to the accumulation of aberrant proteins, which in theory could be reduced by autophagy 16

The membrane was incubated for 1 h in blocking buffer composed of Tween-Tris buffered saline (TTBS, 100 mM Tris, pH 7.5, 150 mM NaCl, 0.2% Tween 20) and 5% nonfat dry milk at 37C

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