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GI side effects nearly doubled when participants jumped straight to higher doses instead of titrating from the lowest starting dose

Cells were transfected at 80% confluency using Lipofectamine 2000 (Thermofisher) Briefly, 2 g pcDNA3.2- aptf2::V5 was diluted in 200 l Opti-MEM (Life Technologies) and 8 l Lipofectamine was diluted in 200 l Opti-MEM

Formulations were diluted 1:100 (v/v) with purified water or fasted-state simulated gastric fluid (FaSSGF, SI Table S4) and droplet size, polydispersity index (PDI) and zeta potential (NanoBrook 90 Plus PALS

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Moreover, T2D promotes the generation of neurotoxic advanced glycation end-products, which are implicated in the pathogenesis of AD (Xia et al