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CSF was applied neat and incubated 24 hours, wells were washed with PBS and a 2 nM mix of PS129 conjugated to Alexa Fluor 488 and Alexa Fluor 647 (ThermoFisher, A20181/6) applied for 3 hours, rinsed and imaged on an ONI Nanoimager using a TIR angle of 53.5

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Telemedicine prescribing has expanded access to GLP-1 medications but varies in quality and cost