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This pulsatile pattern is central to GH biology and is shaped by GHRH, somatostatin, ghrelin, sleep, age, and metabolic state. One important finding from CJC-1295 with DAC research is that GH secretion remained pulsatile even during prolonged GHRH analog stimulation. This helps distinguish GHRH analogs from direct GH replacement, which bypasses upstream pituitary signaling