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aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF + aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 "10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 "10 M Dihexa (Harding lab) in aCSF, 10 "12 M Dihexa in aCSF or 50 ng/ml HGF + 10 " ' 2 M Dihexa in aCSF for 30 minutes at 37C with gentle rocking

Cost-sharing obligations present secondary challenges even after coverage approval

Dont hesitate to call your doctor if youre feeling off or if your lab results show concerning changes

Chiang CK, Wang CC, Lu TF, Huang KH, Sheu ML, Liu SH, et al

For each developmental period, a specific strategy may be considered