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Transformation assays in vitro Agar was prepared in RPMI medium supplemented with 10% heat-inactivated FBS, L-glutamine, penicillin and streptomycin

Yet, the heterogeneity of existing assay characteristics and lack of comprehensive reference values, combined with previously inadequate calibration standards, together with cumbersome correction factors required to compare values between studies obtained with different assay kits, continue to affect the current unsatisfactory situation with IGF-1 testing

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Whether these molecules will become game changers is a question not likely to be answered until the first ones hit the market