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Halophilic characterization of starch-binding domain from Kucuria varient -amylase

198 They also demonstrated that kaempferol exposure to Ishikawa cells inhibited cell migration and invasion by using scratch assay (wound healing) and perforation assay

Tissues were then incubated in 10, 20, and 30% sucrose in 1 PBS, embedded in OCT compound (Tissue-Tek, Sakura Finetek, 4583), snap frozen on dry ice, and then stored frozen at 80C