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"There could be some serious side effects associated with it," he said

Antibodies used for Western blotting included anti-insulin receptor subunit (C-19), anti-IGF-1R subunit (C-20) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and non-immune rabbit serum IgG from Alpha Diagnostic (San Antonio, TX, USA)

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Triplicate measurements per sample is recommended to obtain accurate data