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Deficiency in dietary intakes of methyl donors such as methionine, choline, and folic acid are investigated for their possible effects on NAFLD development 3

This was an adaptation of the protocol described by Neilson and collaborators ( In our case, having used a medium with poor ingredients, with respect to the protocol described in literature ( in vivo study was performed in two phases, first using a wild-type mouse ( Mus musculus ) model, C57BL/6 (FMO3 +/+ ) female mice, where TMAO levels in the blood were measured 3 h after the administration of the inhibitor and 2 hours after the administration of choline orally

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Moreover, it was calculated (based on data by Berkich et al., 2011) sufficed to produce enough aralar for the proposed model to function, although malateaspartate cycle activity needed for synthesis of -ketoglutarate at the beginning of the process increase demands

This was funded by Eli Lilly, India