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Medium was replaced with Seahorse XF Base Medium (Agilent) supplemented with 15 mM glucose (Gibco, A2494001), 1 mM sodium pyruvate (Gibco, 11360070), and 4 mM L-glutamine (Gibco, 25030081), pH 7.4, unless indicated otherwise and incubated in a non-CO2 incubator at 37 C for less than one hour

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Maintaining adequate protein intake and regular resistance exercise remains important for supporting muscle preservation

It also plays a role in how your body uses fat, produces energy, and recovers after physical activity